Naslov
WT-1 Gene Expression in Patients with De Novo Acute Myeloid Leukemia

Autori
Schmid, Daniela ; Tišljar, Kristine ; Linnerth, Bernard ; Priglinger, S. ; Kušec, Rajko ; Sillaber, Christian ; Laczika, K. ; Mitterbauer, Margit ; Pirker, R. ; Zöchbauer, S. ; Lechner, K. ; Jäger, U. ; Gaiger, Alex

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
British Journal of Hematology / Peake, Ian - : Wiley-Blackwell, 1996, 342-342

Skup
Second Meeting of the European Haematology Association

Mjesto i datum
Pariz, Francuska, 29.05.1996. - 01.06.1996

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
WT-1 gene; leukaemia

Sažetak
The Wt-1 gene is a tumor suppressor gene that plays a key role in the pathogenesis of Wilm's tumor. It is located on chromosome 11p13 and encodes a zinc finger transcription factor involved in the regulation of growth and differentiation. Its expression was shown during embryonic development in various tissues as well as in a few human malignancies. Recently, the expression of Wt-specific mRNA in leukemia derived cell lines and cases of acute leukemia was reported, controversial data was obtained regarding the possible prognostic relevance of the Wt-gene expression in patients with acute myelogenous leukemia (AML). The present study was designed to investigate the potential of Wt-1 gene expression as a prognostic factor for AML. We have examined the Wt-1 gene expression in bone marrow samples of 130 patients with de novo AML by two step RT-PCR. The assay allows detection of 1:10Č2 by first step and 1:10Č4 Wt-1 positive cells by second step PCR. Wt-1 gene expression was observed in 76% of the AML patients. We could not observe any significant correlation between the relative levels of Wt-gene expression and the prognosis of acute leukemia, rates of complete remission (CR), disease free survival, overall survival as well as age, WBC, FAB type and cytogenetics at diagnosis. We conclude that in our group of patients Wt-1 PCR positivity is not a prognostic factor in AML. Its expression in the majority of AML patients qualifies the Wt-1 gene transcript as a possible AML marker probably useful in monitoring minimal resudual disease after chemotherapy. Further studies are on the way to evaluate the impact of Wt-1 PCR for the monitoring of minimal residual disease in AML patients.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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