Naslov
Uterine Selection of Human Embryos at Implantation

Autori
Brosens, J.Jan ; ... ; Šućurović, Sandra ; Mulac-Jeričević, Biserka ; ... ; Macklon, Nick S.

Izvornik
Scientific reports (2045-2322) 4 (2014); 3894-1

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
embryo; implantation; human

Sažetak
Human embryos frequently harbor large-scale complex chromosomal errors that impede normal development. Affected embryos may fail to implant although many first breach the endometrial epithelium and embed in the decidualizing stroma before being rejected via mechanisms that are poorly understood. Here we show that developmentally impaired human embryos elicit an endoplasmic stress response in human decidual cells. A stress response was also evident upon in vivo exposure of mouse uteri to culture medium conditioned by low-quality human embryos. By contrast, signals emanating from developmentally competent embryos activated a focused gene network enriched in metabolic enzymes and implantation factors. We further show that trypsin, a serine protease released by pre-implantation embryos, elicits Ca21 signaling in endometrial epithelial cells. Competent human embryos triggered short-lived oscillatory Ca21 fluxes whereas low-quality embryos caused a heightened and prolonged Ca21 response. Thus, distinct positive and negative mechanisms contribute to active selection of human embryos at implantation.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA