Naslov
Apoptosis and sperm DNA fragmentation index in native and processed sperm samples

Autori
Ljiljak, Dejan ; Mahmutefendić H ; Jakovac H, Kuna, Krunoslav ; Trobonjača, Zlatko ; Tramišak Milaković, Tamara ; Smiljan Severinski, Neda ; Radojčić Badovinac, Anđelka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract book / - Sevilla, 2013, 69-69

Skup
5th International IVI Congress: reproductive Medicine and Beyond.

Mjesto i datum
Sevilla, Španjolska, 04.04.2013. - 06.04.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
apoptoza; DNA fragmentacija; tehnike pripreme sjemena
(apoptosis; DNA fragmentation; sperm preparation technique)

Sažetak
Sperm preparation techniques are an essential component of assisted reproductive technologies, and they are related to the success of infertility treatment. The aim of this study was to compare apoptosis and sperm DNA fragmentation between native and processed (after density gradient) sperm samples, and to explore the relation between apoptosis and DNA fragmentation in both sperm samples. The participants were 30 men undergoing fertility treatments in a public hospital. The results showed significantly higher percentage of apoptosis in native sperm sample than in processed sperm sample. The percentage of DNA fragmentation did not differ between native and processed sperm samples. The relation between apoptosis and DNA fragmentation in processed sperm sample and native sperm sample was very low and did not reach statistical significance. Apoptosis was not significantly associated with the number of normal and fragmented forms of sperm cells and this pattern of relations was the same in both samples. The results showed that the age of patients was positively and significantly correlate to the value of apoptosis in processed sperm sample but not in native sample. In conclusion, the findings suggest that even in processed sperm samples the incidence of DNA fragmentation remains unchanged, although sperm processing results in a lower incidence of apoptosis especially in younger men. These findings point to importance of evaluation of apoptosis in processing sperm in IVF.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA