Naslov
Dry powder form and stability of Pluronic mixed micelles in the biologically relevant media

Autori
Pepić, Ivan ; Lovrić, Jasmina ; Hafner, Anita ; Filipović-Grčić, Jelena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts / Lovrić, Jasmina ; Pepić, Ivan ; Filipović-Grčić, Jelena ; Mrhar, Aleš - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2012, 107-107

ISBN
978-953-6256-69-3

Skup
The 9th Central European Symposium on Pharmaceutical Technology

Mjesto i datum
Dubrovnik, Hrvatska, 20.09.2012. - 22.09.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Pluronic® L121; Pluronic® F127; mixed micelles; dry powder; stability

Sažetak
The mixed L121/F127 micelles were prepared at relatively high concentration of Pluronics (1%, w/w) using direct dissolution or film rehydration method with an external input of energy. The optimal preparation was achieved using the film rehydration method followed by ultrasonication. The size of the obtained mixed micelles was 75 nm (PDI = 0.287). Stability studies were performed at 25ºC and 37ºC and upon dilution in different biologically relevant media. Micelles were stable in phosphate buffered saline (PBS) upon 100-fold dilution and for at least 10 days, and in PBS containing bovine serum albumin upon 10 and 50-fold dilution for at least 48 and 12 hours, respectively. The mixed micelles dispersions were freeze-dried after slow (-70ºC for 3 hours) or fast freezing process (-196ºC for 1 min) and with addition of different type (glucose, sucrose, trehalose and mannitol) and amount (0, 1, 2.5, 5, and 10%, w/w) of cryoprotectant. In conclusion, mixed micelles can be obtained as a powder by freeze-drying using trehalose (5%, w/w) or sucrose (2.5%, w/w) with slow or fast freezing process, respectively and retain their structural integrity (dh and PDI values) upon reconstitution. Moreover, stabilization by this technology prolongs the shelf-life of mixed micelles and allows storage at room temperature.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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