Naslov
Chitosan-coating as a mean to prolong vaginal residence time: Liposomes with clotrimazole

Autori
Jøraholmen, May Wenche ; Vanić, Željka ; Tho, Ingunn ; Škalko-Basnet, Nataša

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
3rd Conference on „Innovation in Drug Delivery: Advances in Local Drug Deliv

Mjesto i datum
Pisa, Italija, 22.09.2013. - 25.09.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Chitosan; Liposomes; Mucoadhesion; Clotrinazole; Vaginal delivery

Sažetak
Purpose: The main objective of this study was the optimization of mucoadhesive liposomes containing clotrimazole destined for topical therapy of vaginal infections. Chitosan-coated liposomes of various sizes were evaluated in in vitro and ex vivo experimnets. The effect of chitosan concentration on systems’ features was tested in respect to mucoadhesiveness of the newly developed system. Methods: Liposomes made of phosphatidylcholine and containing clotrimazole were prepared by the modified film method and sonicated to desired vesicle size. Low molecular weight chitosan was used as coating polymer. In vitro clotrimazole release and ex vivo penetration experiments were performed in Franz diffusion system. Mucoadhesiveness was determined in situ using sheep vaginal tissue. Results: Entrapment of clotrimazole in liposomes of average size of 200 nm resulted in 20 % drug yield. The chitosan-coating of vesicles increased the original liposomal size, with higher concentration of polymer (0.6 % ; w/v) resulting in larger vesicles than lower concentration (0.1 % ; w/v). In vitro drug release through artificial membrane indicated that vesicle size and chitosan-coating affect the drug release, with coated liposomes exhibiting slower release. All liposomes (coated and uncoated) sustained the drug release as compared to drug-in-propylene glycol solution. Ex vivo experiments showed that liposomal clotrimazole penetrates into but not through vaginal tissue. Mucoadhesivness was affected by both polymer concentration used for liposome coating, as well as vesicle size. Conclusion: Chitosan-coated liposomes were able to sustain release of clotrimazole and assure needed mucoadhesivness. The fact that liposomal drug was not able to penetrate through vaginal tissue indicate its potential in topical therapy of vaginal infections in pregnant patients.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA