Pregled bibliografske jedinice broj: 678262
In situ hybridization for detection of chicken anaemia virus in peripheral blood smears
In situ hybridization for detection of chicken anaemia virus in peripheral blood smears // Molecular and cellular probes, 11 (1997), 2; 135-141 doi:10.1006/mcpr.1996.0096 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 678262 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
In situ hybridization for detection of chicken anaemia virus in peripheral blood smears
Autori
Novak, Renata ; Ragland, William L.
Izvornik
Molecular and cellular probes (0890-8508) 11
(1997), 2;
135-141
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
chicken anaemia virus; nucleic acid probe; polymerase chain reaction; in situ hybridization; blood smears
Sažetak
Blood smears, obtained from chickens known to be infected with or free from chicken anaemia virus (CAV), were tested to detect CAV DNA by in situ hybridization (ISH). A double-stranded, 1485 base- pair (bp) DNA probe was prepared employing polymerase chain reaction (PCR). The probe was labelled with digoxigenin or biotin by nick translation. In situ hybridization employing simple specimen preparation, using both digoxigenin- and biotin-labelled probes, provided a rapid and inexpensive method for diagnosis of CAV infection. Infection with CAV was detected by ISH prior to seroconversion by indirect immunofluorescence assay.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Veterinarska medicina
POVEZANOST RADA
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
