Naslov
What is the biological role of paralogous SSBs in Streptomyces coelicolor?

Autori
Vujaklija, Dušica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
Universite de Pau et des Pays de l'Adour, Equipe Environnement et Microbiologie, UMR CNRS 5254, IBEAS - UFR Sciences et Techniques

Mjesto i datum
Pau, Francuska, 21.12.2012

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
Streptomyces coelicolor ; SSB

Sažetak
The linear chromosome of Streptomyces coelicolor contains two paralogous genes encoding single- stranded DNA binding proteins, ssbA and ssbB. The functions of these SSBs (SSB-A and SSB-B) have not been studied so far. By disrupting these genes we have shown that ssbA gene is indispensable for cell viability, while ssbB participate in genome segregation during sporulation. Mutant phenotype has been restored by the introduction of single copy of ssbB gene. We also demonstrated differential expression of two ssb genes in S. coelicolor. During bacterial growth, gene ssbA is constantly expressed at higher level ; its expression is decreasing towards late stationary phase, while the expression of ssbB is at all-time low. In minimal medium the ssbB gene is significantly up regulated. Promoters for both genes are determined. Surprisingly, promoter of ssbB gene possesses unusual features and is active in taxonomically distant bacteria Escherichia coli.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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