Pregled bibliografske jedinice broj: 675281
The Use of Mass Spectrometry in Characterization of Bone Morphogenetic Proteins from Biological Samples
The Use of Mass Spectrometry in Characterization of Bone Morphogenetic Proteins from Biological Samples // Applications of Tandem Mass Spectrometry / Prasain, Jeevan (ur.).
Rijeka: In Tech, 2011. str. 259-284
CROSBI ID: 675281 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The Use of Mass Spectrometry in Characterization of Bone Morphogenetic Proteins from Biological Samples
Autori
Razdorov, Genadij ; Vukicevic, Slobodan
Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, stručni
Knjiga
Applications of Tandem Mass Spectrometry
Urednik/ci
Prasain, Jeevan
Izdavač
In Tech
Grad
Rijeka
Godina
2011
Raspon stranica
259-284
ISBN
978-953-307-870-0
Ključne riječi
Mass Spectrometry, Bone Morphogenetic Proteins, BMPs
Sažetak
Growth factors MS based characterization from biofluids is almost exclusively done by CID on plethora of different tandem instruments. Very low levels of growth factors in circulation is limiting their MS based identification due to huge dynamic range of plasma/serum proteins. Different biochemical purification strategies are used to enrich individual growth factors enough to be able to analyse it by MS. Most of them use heparin affinity chromatography as one purification step. We have successfully identified number of growth factors from biofluids using heparin based enrichment, followed by the SDS-PAGE protein separation and peptide analysis on nano LC coupled to LTQ-Orbitrap.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
108-1080327-0320 - Uloga TSH u modelu osteoporoze i u bolesnica sa smanjenom koštanom masom (Vukičević, Slobodan, MZOS ) ( CroRIS)
Ustanove:
Medicinski fakultet, Zagreb
