Pregled bibliografske jedinice broj: 67445
THE ROLE OF PKC, PI3-KINASE AND PHOSPHOTYROSINE PHOSPHATASE IN INSULIN ACTION AND INSULIN RESISTANCE
THE ROLE OF PKC, PI3-KINASE AND PHOSPHOTYROSINE PHOSPHATASE IN INSULIN ACTION AND INSULIN RESISTANCE // International Conference on Signal Transduction, Abstracts / Đikić, I ; Husnjak, K ; Pavelić, J ; Pavelić, K (ur.).
Zagreb, 2000. (poster, međunarodna recenzija, sažetak, znanstveni)
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Naslov
THE ROLE OF PKC, PI3-KINASE AND PHOSPHOTYROSINE PHOSPHATASE IN INSULIN ACTION AND INSULIN RESISTANCE
Autori
Roša, Jagoda ; Roša, Josip
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
International Conference on Signal Transduction, Abstracts
/ Đikić, I ; Husnjak, K ; Pavelić, J ; Pavelić, K - Zagreb, 2000
Skup
2nd international Conference on Signal Transduction
Mjesto i datum
Dubrovnik, Hrvatska, 26.05.2000. - 31.05.2000
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
hepatocytes; insulin signal; insulin resistance; PKC; PI3-kinase; Phosphotyrosine phosphatase
Sažetak
The aim of this investigation was to determine the possible role of PKC, PI3-kinase and phosphotyrosine phosphatase in insulin signal patway and insulin resistance. Hepatocytes were isolated by a modified collagenase perfusion technique of Berry and Friend and cultured for 72 h in the medium containing either low (5 mmol/l) or high (25 mmol/l) glucose without or with (0.08 umol/l)insulin. For study AIB transport cells were incubated for one hour in hanks-hepes medium containing 2 g/l albumin and alfa-amino isobutiric acid in the absence and presence of insulin. PKC stimulator (TPA)or inhibitor polimixin B, PI3-kinase inhibitor wortmannin and phosphotyrosine phosphatase inhibitor vanadate were added to the medium during transport assay. Results: hyperglycemia decreased basal and insulin-stimulated AIB transport. Activation of tyrosine kinase and PKC produced insulin like effect. Inhibition PI3-kinase decreased insulin effect. Activation of tyrosine kinase and PKC reversed toxic effect of glucose on basal transport. Inhibition of PKC reversed insulin action on AIB transport in hepatocytes incubated in high glucose. In conclusion, the effect of insulin on AIB transport in hepatocytes occurs through activation of tyrosine kinase, protein kinase C and PI3-kinase. Hyperglycemia through PKC activation cause insulin resistance.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
