Inhibitory effect of a propolis on Di-n-Propyl Disulfide or n-Hexyl salycilate-induced skin irritation, oxidative stress and inflammatory responses in mice

Oršolić, Nada; Skurić, Jadranka; Đikić, Domagoj; Stanić, Gabrijela

doi:10.1016/j.fitote.2013.12.007

Pregled bibliografske jedinice broj: 673083

Inhibitory effect of a propolis on Di-n-Propyl Disulfide or n-Hexyl salycilate-induced skin irritation, oxidative stress and inflammatory responses in mice

Oršolić, Nada; Skurić, Jadranka; Đikić, Domagoj; Stanić, Gabrijela

Inhibitory effect of a propolis on Di-n-Propyl Disulfide or n-Hexyl salycilate-induced skin irritation, oxidative stress and inflammatory responses in mice // Fitoterapia, 93 (2014), 18-30 doi:10.1016/j.fitote.2013.12.007 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 673083 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Inhibitory effect of a propolis on Di-n-Propyl Disulfide or n-Hexyl salycilate-induced skin irritation, oxidative stress and inflammatory responses in mice

Autori
Oršolić, Nada ; Skurić, Jadranka ; Đikić, Domagoj ; Stanić, Gabrijela

Izvornik
Fitoterapia (0367-326X) 93 (2014); 18-30

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Mouse models of psoriasis; Skin lesions; Propolis; Lipid peroxidation; Glutathione level; Thermography

Sažetak
Purpose Thermal imaging has been utilised, both preclinically and clinically, as a tool for assessing inflammation. Psoriasis is a chronic inflammatory skin disease characterised by hyperkeratosis, dermal inflammatory infiltrate and increased angiogenesis. The aim of the present study was to assess the usefulness of thermography in psoriatic lesion regression after topical treatment with bee propolis, recognised as potent antioxidants and anti-inflammatory agents. Methods We monitored the inflammation process induced by irritants such as n-Hexyl salycilate (HXS) or Di-n-Propyl Disulfide (PPD) by histopatological assessment of the skin, thermographic scanning, total number of inflammatory cells in the peritoneal cavity, differential analysis of cells in the peritoneal cavity, macrophage spreading index, haematological and biochemical parameters, frequencies of micronucleated reticulocytes, lipid peroxidation and glutathione assay in the skin. Results Topically applied ethanolic extract of propolis (EEP) with HXS or PPD reduced the lipid peroxidation in the skin and total number of inflammatory cells in the skin and peritoneal cavity, functional activity of macrophages, the number of micronuclei in mouse peripheral blood reticulocytes and enzymatic activity of ALP and AST. Conclusion These results demonstrate that topical application of EEP may improve psoriatic-like skin lesions by suppressing functional activity of macrophages and ROS production. Taken together, it is suggested that EEP can safely be utilised in the prevention of psoriasis-related inflammatory changes without causing any toxic effect.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Farmacija

POVEZANOST RADA

Projekti:
119-0000000-1255 - Kemoprevencija rasta tumora polifenolnim sastavnicama (Oršolić, Nada, MZOS ) ( CroRIS)
119-0532265-1254 - Imunoadjuvantno i zaštitno djelovanje propolisa u životinja (Benković, Vesna, MZOS ) ( CroRIS)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb,
Klinička bolnica "Sveti Duh"

Profili:

Domagoj Đikić (autor)