Naslov
Antimicrobial activity of modified cell penetrating peptide and modified natural antimicrobial peptide against opportunistic (multiresistant) pathogens in hospital environment

Autori
Vučemilović-Alagić, Nataša

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Prirodoslovno-matematički fakultet u Splitu

Mjesto
Split

Datum
04.10

Godina
2013

Stranica
50

Mentor
Juretić, Davor

Neposredni voditelj
Simunić, Juraj

Ključne riječi
antimicrobial resistance; antimicrobial peptides; Acinetobacter baumannii

Sažetak
Antimicrobial resistance is a problem that occurs when the antibiotic loses the ability to control or inhibit the growth of bacteria. The bacteria continue to multiply in spite of therapeutic drug concentrations. Studies have shown that antimicrobial resistance is increasing, and it is necessary to create a new mode of action against pathogens. The aim of this thesis was to find a minimum inhibitory concentration (MIC) for certain strains of bacteria that were treated with diluted antimicrobial peptides. The selected group of bacteria causes most infections in immunocompromised patients and in hospital settings. Antimicrobial peptides used in the experimental part of this thesis have different backgrounds. MAPL6A is the computer-modified peptide cell-penetrating peptide. DAP is a hybrid peptide made as seqential dimer of weakly active natural amphibian peptide. One amino acid substitution was introduced into sequential dimmer, because the Mutator algorithm (http://split4.pmfst.hr/mutator/) suggested that it will increase its selectivity against Gram- negatives. The hospital laboratory measurements were performed on resistant and sensitive strains. Eight strains have been tested of which five are Gram-negative and three are Gram-positive. MIC values are less than 10 micrometers for almost all strains. Strains with MIC value greater of 10 μM are: E. coli ATCC (12μM) and MRSA (25μM) for DAP and K. pneumoniae for MAPL6A (12μM). As the most important value of MIC, we highlight 3 μM (DAP) for multiresistant A. baumannii and P.areuginosa. A. baumannii is considered to be the most difficult to destroy in hospitals.The results encourage additional work in order to find the best possible peptide (or combinations of peptide antibiotics with other antibiotics) to deal with the one of the most pressing problems in the hospitals, the emergence of pan-resistant bacterial pathogens.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija, Temeljne medicinske znanosti

Napomena
Magistarski rad (MSc thesis)

POVEZANOST RADA