Naslov
Bubbles, microparticles, and neutrophil activation: changes with exercise level and breathing gas during open-water SCUBA diving

Autori
Thom, Stephen R. ; Milovanova, Tatyana N. ; Bogush, Marina ; Yang, Ming ; Bhopale, Veena M. ; Pollock, Neal W. ; Ljubković, Marko ; Denoble, Petar ; Madden, Dennis ; Lozo, Mislav ; Dujić, Željko.

Izvornik
Journal of applied physiology (8750-7587) 114 (2013), 10; 1396-1405

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
SCUBA diving; microparticles; decompression stress

Sažetak
The study goal was to evaluate responses in humans following decompression from open-water SCUBA diving with the hypothesis that exertion underwater and use of a breathing mixture containing more oxygen and less nitrogen (enriched air nitrox) would alter annexin V- positive microparticle (MP) production and size changes and neutrophil activation, as well as their relationships to intravascular bubble formation. Twenty-four divers followed a uniform dive profile to 18 m of sea water breathing air or 22.5 m breathing 32% oxygen/68% nitrogen for 47 min, either swimming with moderately heavy exertion underwater or remaining stationary at depth. Blood was obtained pre- and at 15 and 120 min postdive. Intravascular bubbles were quantified by transthoracic echocardiography postdive at 20- min intervals for 2 h. There were no significant differences in maximum bubble scores among the dives. MP number increased 2.7-fold, on average, within 15 min after each dive ; only the air-exertion dive resulted in a significant further increase to 5-fold over baseline at 2 h postdive. Neutrophil activation occurred after all dives. For the enriched air nitrox stationary at depth dive, but not for other conditions, the numbers of postdive annexin V-positive particles above 1 μm in diameter were correlated with intravascular bubble scores (correlation coefficients ∼0.9, P < 0.05). We conclude that postdecompression relationships among bubbles, MPs, platelet- neutrophil interactions, and neutrophil activation appear to exist, but more study is required to improve confidence in the associations.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA