Naslov
Cag A tipiziranje kliničkih izolata H. pylori : naša prva iskustva
(Cag A typing of H. pylori clinical isolates : our first experiences)

Autori
Plečko, Vanda ; Rebrović, B. ; Katić, S. ; Katičić, Miroslava ; Presečki, Vladimir ; Dominis, Mara

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Drugi kongres Hrvatskog gastroenterološkog društva = 2nd Congress of the Croatian Society of Gastroenterology / Vucelić, Boris - Zagreb : Medicinska naklada, 1997, 193-193

Skup
Drugi kongres Hrvatskog gastroenterološkog društva s međunarodnim sudjelovanjem

Mjesto i datum
Zagreb, Hrvatska, 01.10.1997. - 03.10.1997

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Helicobacter pylori; Cag A; PCR; molekularna tipizacija
(Helicobacter pylori; Cag A; PCR; molecular typing)

Sažetak
The importance od H. pylori infection in pathogenesis od duodenal ulcer disease in recognised by many studies. Variability in clinical symptom of infected persons might be explained with differences in immunological responses and in different pathogenecity of H. pylori strains. Cag A gen is thought to be important virulence marker, among other putative factors. Samples. We tested 9 clinical isolates of H. pylori isolated from patients visiting KB Merkur with peptic ulcer disease as well as standard strain (H. pylori ATCC 43504). Method. For detection of cag A gen we used "in house" PCR method. 5 ľl chomosomal DNA isolated with standard procedures, was mixed with 95 ľl master mix. PCR amplification was performed in Perkin-Elmer thermocycler 9600. The oligonucleotides used as PCR primers for detection of cag A gen were D008 and R008. PCR product were electophoresed on a 1.2% agarose gel. Positive samples gave PCR product of 298 bp. We used standard strain as a positive control, master mix without DNA as a negative control and DNA 123 bp marker. Results. We typed H. pylori clinical isolate cultivated from gastric biopsies isolated at the end of 1996 and during 1997. These 9 isolates were from patients presenting with upper gastroduodenal symptoms, 2 patients had clinical diagnosis of gastric ulcer, 7 duodenal ulcers, 1 patient had erosions and duodenal ulcer. Cag A typing gave 8 positive and 1 negative results. Cag A negative strain was from patient with erosions and duodenal ulcer. Serological tests (CF, and 1 patient's ELISA) were positive in all patients. Conclusion. Our first experiences in typing H. pylori obtained from patients with clinical important infections are in agreement with literature. It is important to continue Cag A typing and other molecular typing on a greater number of H. pylori strains. Then we will be able to compare this pathogenicity marker of our strains with clinical signs and symptoms in patients.

Izvorni jezik
Hrvatski

Znanstvena područja
Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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