Naslov
Aqueous Two-Phase Extraction of Trametes Versicolor Laccase Within Microfluidic Device: Experiments and Modeling

Autori
Novak, Uroš ; Nujić, Marija ; Tišma, Marina ; Žnidaršič-Plazl, Polona ; Plazl, Igor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Skup
2013 AIChE Annual Meeting

Mjesto i datum
San Francisco (CA), Sjedinjene Američke Države, 03.11.2013. - 08.11.2013

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Aqueous Two-Phase Extraction; Microreactor; Modelling

Sažetak
ATPS offers several advantages compared to traditional extraction with organic solvents, particularly regarding to the non-denaturing conditions and high biocompatibility The polyethylene glycol (PEG)/phosphate aqueous two- phase system (ATPS) was used for extraction of commercial Trametes versicolor laccase within a Ψ- branched inflow and outflow glass microchannel. The flow rates of both phases entering Ψ-branched were selected in order to enable the separation of phases at the exit of microchannels in microfluidic device. Due to decreased scales in Ψ- branched microchannel, the extraction of laccase from phosphate-rich phase to PEG-rich phase reached equilibrium with the highest obtained extracted fraction of laccase of 65 % (w/w). The diffusion coefficients for laccase in both phases were determined based on the experimental data obtained in a short Y-junction microfluidic chip and the corresponding mathematical model. Furthermore, a 3D mathematical model in dimensionless form was developed comprising convection and diffusion terms at steady state conditions and it was validated by experimental results in order to forecast the performance for a wide range of flow rates.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

POVEZANOST RADA