Naslov
Hippocampal and cortical neuronal damage in the streptozotocin rat model of Alzheimer’s diseases

Autori
Knapić, Marina ; Knezović, Ana ; Šimić, Goran ; Šalković-Petrišić, Melita

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Clinical Neuropathology 31(4) / Ironside, James - Edinburgh : British Neuropathological Society, 2012, 290-290

Skup
10th European Congress of Neuropathology

Mjesto i datum
Edinburgh, Ujedinjeno Kraljevstvo, 06.06.2012. - 09.06.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
hippocampus; streptozotocin; neuron damage

Sažetak
Introduction: Morphological changes in the brain of the streptozotocin-intracerebroventricularly (STZ-icv) treated rat model of sporadic Alzheimer’s disease (sAD) have been investigated in much lesser extent than the behavioral and neurochemical ones. We have investigated the STZ-icv dose-dependency of rat cortical and hippocampal neuronal damage in the acute and chronic phase following the icv-treatment. Methods: Adult male Wistar rats were administered STZ (0.1, 1 and 3 mg/kg dose) or vehicle (controls) icv injections and sacrificed 1 week and 3 months afterwards. Paraffin-embedded brain sections of pre-mortally fixative perfused animals were analysed by Nissl staining in the parietal cortex (PC), hippocampal regions C1, C2 and dentate gyrus (DG). Positive signal was image analyzed and quantified by “CellSense Dimension”. Statistical significance was tested by Kruskal-Wallis followed by Mann Whitney U test, p<0.05. Results: Nissl staining revealed acute changes in the hippocampal regions only, manifested generally as 25% decrease in cell number in C1 and DG with the highest dose (p<0.05). Three months after the treatment, these values were normalized in comparison to the control, while slight tendency to decrease (-16%) has been found in the cell number in the PC region (p<0.05). Conclusions: These preliminary results suggest that neuronal damage seems to be manifested in the post-treatment- and region-dependent manner in the STZ-icv rat model of sAD, with no particular STZ-icv dose dependency. Acknowledgement: Supported by the Unity Through Knowledge Fund (UKF 64-10) and MZOS

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA