Naslov
Amyloid-β pathology in the brain of a streptozotocin rat model of sporadic Alzheimer’s disease

Autori
Šalković-Petrišić, Melita ; Knezović, Ana ; Knapić, Marina ; Šimić, Goran

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Clinical Neuropathology 31(4) / Ironside, James - Edinburgh : British Neuropathological Society, 2012, 290-290

Skup
10th European Congress of Neuropathology

Mjesto i datum
Edinburgh, Ujedinjeno Kraljevstvo, 06.06.2012. - 09.06.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
amyloid-beta; streptozotocin; angiopathy

Sažetak
Introduction: Streptozotocin-intracerebroventricularly (STZ-icv) treated rats represent experimental non-transgenic model of sporadic Alzheimer´s disease (sAD) which develop cerebral amyloid pathology in the form of β-amyloid aggregation in the blood vessel wall, found three months after icv administration of 3 mg/kg STZ dose. We have investigated the STZ-icv dose-dependency of β-amyloid pathology development 1 week and 3 months after the STZ-icv treatment. Methods: Adult male Wistar rats were administered STZ (0.1, 1 and 3 mg/kg dose) or vehicle (controls) icv injections and sacrificed 1 week and 3 months afterwards. Paraffin-embedded brain sections of pre-mortally fixative perfused animals were analysed by Congo Red and Bielschowsky Silver staining and Aβ1-42 immunohistochemistry. Results: Congo Red staining revealed β-amyloid aggregation in the meningeal capillaries only with the highest STZ-icv dose 3 months following the treatment with no specific signal being found with lower doses and at 1 week time-point. This finding has been supported by some positive Bielschowsky Silver staining signal in the blood vessel wall. No plaque-like formations of positive Aβ1-42 aggregation with any of the dose could have been detected by immunohistochemistry at both time-points. Conclusions: Development of cerebral β-amyloid pathology in the STZ-icv rat model of sAD is a dose-dependent, non-acute effect which takes at least 3 months to be manifested in the form of angiopathy. Acknowledgement: Supported by the Unity Through Knowledge Fund (UKF 64-10) and MZOS.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA