Naslov
VALIDATION OF HPLC ASSAY FOR THE DETERMINATION OF OXYPRENYLATED ANTHRAQUINONES PRODUCED BY RHAMNUS L. SPECIES

Autori
Locatelli, Marcello ; Carlucci, Giuseppe ; Epifano, Francesco ; Kremer, Dario ; Genovese, Salvatore

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
8th Joint Meeting of AFERP, ASP, GA, PSE and SIF: Congress Abstracts u Planta Medica 78(2012) / O'Neil-Johnson - , 2012, PJ6-PJ6

Skup
8th Joint Meeting of AFERP, ASP, GA, PSE and SIF

Mjesto i datum
New York City (NY), Sjedinjene Američke Države, 28.07.2012. - 01.08.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Rhamnus; anthraquinones; HPLC

Sažetak
Rhamnus L. spp. (Rhamnaceae) is known to contain active anthraquinones secondary metabolites but the presence of oxyprenylated ones is still not reported. As continuation of our studies [1, 2], a new method [3] was validated to quantify these analytes in plant extracts after extraction with n-hexane and methanol using a ODS column, water and methanol (1% formic acid, v/v) as mobile phase at 0.7 mL min-1 in gradient elution. Quantitative analyses, at 435 nm, revealed LOQ of 0.5 M and linearity up to 125 M. Precision values ranged from 0.2% to 12.9% while trueness from 12.2% to 12.7%.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Farmacija, Šumarstvo

POVEZANOST RADA