Naslov
Comparative performance of HIV-1 RNA load assays at low viral load levels : Results of an international collaboration

Autori
Swenson, Luke ; Cobb, Bryan ; Geretti, Anna ; Harrigan, Richard ; Poljak, Mario ; Seguin-Devaux, Carole ; Verhofstede, Chris ; Wirden, Marc ; Amendola, Alessandra ; Boni, Jurg ; Bourlet, Thomas ; Huder, Jon ; Karasi, Jean-Claude ; Židovec Lepej, Snježana ; Lunar, Maja ; Mukabayire, Odette ; Schuurman, Rob ; Tomažič, Janez ; Van Laethem, Kristel ; Vandekerckhove, Linos ; Wensing, Anne

Izvornik
Journal of clinical microbiology (0095-1137) 52 (2013), 2; 517-23

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
antiretroviral therapy; HIV-1; viraemia

Sažetak
Low-level viraemia during antiretroviral therapy and its accurate measurement have become increasingly relevant. We present an international collaboration of 4, 221 paired plasma viral load (pVL) results from four commercial assays, emphasizing data with low pVL.The assays compared were the Abbott RealTime assay and the Roche Amplicor, TaqMan v1 and v2 assays. Correlation between assays was 0.90-0.97. However, at low pVL, correlation fell to 0.45-0.85. Higher inter-assay concordance was observed when detectability was defined as 200-copies/mL versus 50-copies/mL. A pVL of ∼100-125 copies/mL by TaqMan v1/v2 corresponded best to a 50-copies/mL threshold by Amplicor.Correlation and concordance between viral load assays were lower at low pVL. Clear guidelines are needed on the clinical significance of low-level viraemia.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA