Naslov
PROCJENA GENOTOKSIČNOG UČINKA KADMIJA I BAKRA U DUHANU METODOM RAPD
(CADMIUM- AND COPPER- INDUCED GENOTOXICITY BY RANDOM AMPLIFIED POLIMORPHIC DNA IN TOBACCO)

Autori
Drempetić, Ana

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Prirodoslovno-matematički fakultet

Mjesto
Zagreb

Datum
06.07

Godina
2013

Stranica
39

Mentor
Pavlica, Mirjana

Neposredni voditelj
Cvjetko, Petra

Ključne riječi
Nicotiana tabacum L. / bakar / kadmij / genotoksičnost / metoda RAPD / bioakumulacija
(Nicotiana tabacum L. / copper / zinc / cadmium / genotoxicity / random amplified polimorphic DNA / bioacumulation)

Sažetak
Heavy metals are persistent environmental pollutants, and can have toxic effects due to their accumulation in living organisms. Effect of heavy metals at different levels of biological organization is generally well known, but the combined effect of several heavy metals on the organism has been insufficiently studied. The genotoxic effect of cadmium and copper, alone and in combination, in tobacco Nicotiana tabacum L., was investigate in this study. The aim was to determine the polymorphism of DNA as a result of tobacco exposure to heavy metals cadmium and copper, and evaluate if the combined effect of these metals is synergistic or antagonistic. Metal content analysis revealed that the influence of copper and cadmium depended on the concentration and developmental stage of plants. Copper increased toxic effects of cadmium in the seedlings (synergistic effect), whereas in adult plants reduce toxic effects of cadmium (antagonistic effect). Seedlings have reduced genome stability in comparison to adult plants.

Izvorni jezik
Hrvatski

Znanstvena područja
Biologija

POVEZANOST RADA