Naslov
The antimicrobial effect of photodynamic therapy used as an adjunct to conventional endodontic retreatment: a clinical study

Autori
Bago Jurič, Ivona ; Plečko, Vanda ; Gabrić Pandurić, Dragana ; Baraba, Anja ; Anić, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
The 4th Congress of the World Federation for Laser Dentistry European Division

Mjesto i datum
Bruxelles, Belgija, 10.07.2013. - 13.07.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
root canal; photodynamic therapy; endodontics

Sažetak
Aim To evaluate the efficacy of antimicrobial photodynamic therapy (aPDT) used as an adjunct to the endodontic retreatment in the eradication of microorganisms from previously filled root canals. Methodology The study sample consisted of 21 randomly selected patients with chronic periapical periodontitis on incisors or canines, who have had previously endodontic treatment, more than two years ago. Microbiological samples from the root canals were collected after accessing the canal, following the endodontic retreatment and after the aPDT procedure. Twenty one teeth were retreated, instrumented with ProTaper files. After each instrument, the root canals were irrigated with 2, 5% sodium hypochlorite (NaOCl), and the final irrigation protocol included 17% ethylenediaminetetraacetic acid followed by NaOCl and saline solution. Root canals were filled with a photosensitizing agent, phenothiazine chloride, which was after 2 min removed with distilled water. Then, the root canals were irradiated with a 3D Endoprobe of a diode laser (λ=660 nm, 100 mW) for 1 min. Microbiological samples from the root canals were cultivated on selective plates, and the identification was done by micromorphology, macromorphology and different API strips every 48 hours during 14 days. Results Fourteen bacteria species were isolated from root canals initially, with a mean value of 4.57 species per canal. Although endodontic retreatment alone produced a significant reduction in the number of bacteria species (p<0.001), the combination of endodontic treatment and aPDT was statistically more effective (p<0.001), and suceeded in the total elimination of bacteria from 11 root canals. Conclusions The results indicate that the aPDT used as an adjunct to the conventional endodontic therapy achieves a significant futher reduction of intracanal microbial load.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina

POVEZANOST RADA