Naslov
Protein adsorption on various plasma-treated polyethylene terephthalate substrates

Autori
Recek, Nina ; Jaganjac, Morana ; Kolar, Metod ; Milković, Lidija ; Mozetič, Miran ; Stana-Kleinschek, Karin ; Vesel, Alenka

Izvornik
Molecules (1420-3049) 18 (2013), 10; 12441-12463

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
oxygen and fluorine plasma treatment; polymer surface modification; protein adsorption; cell adhesion; quartz crystal microbalance (QCM)

Sažetak
Protein adhesion and cell response to plasma-treated polymer surfaces were studied. The polymer polyethylene terephthalate (PET) was treated in either an oxygen plasma to make the surface hydrophilic, or a tetrafluoromethane CF4 plasma to make the surface hydrophobic. The plasma source was radiofrequency (RF) discharge. The adsorption of albumin and other proteins from a cell-culture medium onto these surfaces was studied using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The cellular response to plasma-treated surfaces was studied as well using an MTT assay and scanning electron microscopy (SEM). The fastest adsorption rate was found on the hydrophilic oxygen plasma-treated sample, and the lowest was found on the pristine untreated sample. Additionally, the amount of adsorbed proteins was higher for the oxygen-plasma-treated surface, and the adsorbed layer was more viscoelastic. In addition, cell adhesion studies support this finding because the best cell adhesion was observed on oxygen-plasma-treated substrates.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA