Naslov
Adaptation of permanent fish cell line CCO for growth in serum-free medium

Autori
Gaurina Srček, Višnja ; Radošević, Kristina ; Dukić, Bogdanka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
European Biotechnology Congress 2013 - Current Opinion in Biotechnology / Van der Meer, Jan ; Stephanopoulos, Greg N. - Bratislava : Elsevier, 2013, S63-S63

Skup
European Biotechnology Congress 2013

Mjesto i datum
Bratislava, Slovačka, 16.05.2013. - 18.05.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
adaptation; CCO cells; serum-free medium

Sažetak
The use of fish cell lines in biotechnology, ecotoxicology, virology and basic researches is constantly increasing. Proliferation of fish cells in culture is dependent on factors usually presented in serum of animal origin. Due to many obstacles regarding serum usage, serum-free media (SFM) are preferred, especially in the bioprocesses using animal cells. The aim of this study was to adapt fish CCO cells to grow in SFM by gradual reduction of serum content. During adaptation in UltraCulture medium (UCM) with 10%-0% (v/v) foetal bovine serum (FBS), obtained cell yields were in the range of 17.8-2.8x10e5 cells/ml. The cryopreserved SFM-CCO cells were checked during 3 months of storage and could be revived with cell monolayer established within one week. Since CCO cells are used for Channel Catfish Virus (CCV) vaccine production, we also attempted to obtain higher cell yields in low-serum media. CCO cells adapted to 2% of FBS were cultivated on Cytodex 1 microcarriers in Cell-spin bioreactors for 13 days. Results showed slightly higher cell yield (17.0x10e5 cells/ml) in UCM with 2% FBS when compared to regular DMEM medium with 5% FBS (15.0x10e5 cells/ml). In conclusion, CCO cells were successfully adapted to grow in SFM but further optimization is needed for their efficient use in microcarriers/bioreactor system.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA