Naslov
Greater osteoclastogenic potential in patients with rheumatoid arthritis for synovial fluid but similar for peripheral blood mononuclear cells compared to patients with spondyloarthritis.

Autori
. Ikić, Marina ; Lazić, Elvira ; Jajić, Zrinka ; Grubišić, Frane ; Mitrović, Hrvoje ; Stipić Marković, Asja ; Kovačić, Nataša ; Marušić, Ana ; Grčević, Danka.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2010 Annual Meeting of the Austrian Society for Allergy and Immunology (OGAI), Book of Abstracts. / - Beč, 2010, 46-46

Skup
2010 Annual Meeting of the Austrian Society for Allergy and Immunology (OGAI)

Mjesto i datum
Beč, Austrija; Zagreb, Hrvatska, 22.12.2010. - 24.12.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
osteoclasts; cytokines; bone loss; arthritis

Sažetak
Three major forms of chronic joint diseases are classified in clinical practice: osteoarthritis (OA), rheumatoid arthritis (RA) and spondyloarthritis (SpA) that includes ankylosing spondylitis (AS) and psoriatic arthritis (PA). Those forms differ in the osteoclast activity and the intensity of cartilage and bone destruction, with RA as a prototype of “destructive” and SpA as a prototype of “remodeling“ arthritis. The aim of our study was to assess the osteoclastogenic potential of peripheral-blood mononuclear cells (PBMC) and local synovial-fluid derived mononuclear cells (SFMC) in patients with RA and SpA. PBMC and SFMC were collected from RA and SpA patients. Osteoclast differentiation was stimulated with M-CSF and RANKL. Osteoclasts (OCs) were detected as TRAP-positive multinucleated cells. RNA was extracted from PBMC/SFMC and osteoclastogenic cultures, reversely trancribed to cDNA, and amplified by quantitative PCR for the expression of osteoclast differentiation genes (RANK, cFms) and inflammatory mediators (MCP-1, TNF-α, IL-17, IL-18, VEGF). In addition, serum and synovial fluid levels of the same mediators were determined by ELISA. Our results showed that PBMC from RA and SpA patients had similar osteoclastogenic potential, whereas SFMC from RA patients generated higher number of OCs compared with SFMC from SpA patients. Additionally, SFMC from RA group showed greater osteoclastogenic potential than PBMC. Greater osteoclastogenic potential in RA was paralleled with the increased expression of osteoclast differentiation genes as well as with higher expression of MCP-1, VEGF and TNF-α. Our results indicate that osteoclast progenitors, capable for differentiation into TRAP-positive cells, are contained among both PBMC and SFMC but not with the same frequency in different forms of arthritis and even within the PBMC and SFMC of the same patient. These findings may be relevant for the development of therapeutic approaches aimed to modulate their action.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA