Naslov
RAPD and rep-PCR genomic fingerprinting of indigenous Bradyrhizobim japonicum strains.

Autori
Sikora, Sanja ; Redžepović, Sulejman

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd Croatian Congress of microbiology with international participation Brijuni. Proceedings. / Prukner-Radovčić, Estella - Zagreb : Hrvatsko mikrobiološko društvo, 2000, 124-124

Skup
2nd Croatian Congress of microbiology with international participation - Brijuni.

Mjesto i datum
Brijuni, Hrvatska, 03.10.2000. - 06.10.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Bradyrhizobium japonicum; RAPD; rep-PCR; fingerprinting; ERIC; BOX

Sažetak
Bradyrhizobium japonicum, a nitrogen-fixing soil bacterium capable to induce formation of nodules on soybean roots, is of great agricultural importance. Since it has been recognized that bradyrhizobial strains can considerably differ in their symbiotic efficiency, suitable methods for strain identification are needed. Genetic diversity of 10 B. japonicum strains isolated from different regions of Croatia was studied. All field isolates as well as reference strain were analysed by using two PCR-based fingerprinting methods - randomly amplified polymorphic DNA (RAPD) and repetitive element sequence based PCR (rep-PCR). Specific and reproducible band patterns were produced in amplification reactions with three oligonucleotide primers of arbitrary sequence as well as with repetitive (ERIC, REP BOX) primers. Although the ERIC primer set generated fingerprints with lower level of complexity, several very clear, intense and discriminative bands were detected which undoubtely divided all isolates into three main groups. The dendrogram derived from RAPD fingerprints showed the same grouping of strains with the higher level of diversity within each group. The most complex fragment pattern was obtained by using BOX primer. The dendrograms derived from REP and BOX fingerprints showed slightly differences within main group of strains. Both RAPD and rep-PCR genomic fingerprints generated from B. japonicum isolates enabled differentiation at the strain level. Our results suggest that for reliable cluster analysis, the most important is to obtain a sufficient number of strong and clear polymorphic bands. The highest level of discrimination and the most reliable characterization of B. japonicum isolates were obtained by using several short oligonucleotide primers in RAPD analysis. The dendrograms derived from combined ERIC, REP and BOX fingerprints revealed the same grouping of B. japonicum strains.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

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