Naslov
Inference of the Population Structure of Leishmania infantum from Southeastern Europe focusing on Cyprus Greece and Turkey

Autori
Gouzelou, Evi ; Haralambous, Christos ; Martinković, Franjo ; Pratlong, Francine ; Dedet, Jean-Pierre ; Antoniou, Maria ; Özensoy, Toze Seray ; Presber, Wolfgang ; Schönian, Gabriele ; Soteriadou, Ketty

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The Fifth World Congress on Leishmaniasis, Abstract Book, / - , 2013, 105-106

Skup
World Leish 5, The Fifth World Congress on Leishmaniasis

Mjesto i datum
Porto de Galinhas, Brazil, 13.05.2013. - 17.05.2013

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
leishmania infantum; population genetics; southeastern europe

Sažetak
Leishmania infantum is the main causative agent of human and canine visceral leishmaniasis (HVL and CanVL, respectively) and also responsible for sporadic cases of cutaneous leishmaniasis (CL) across the Mediterranean. Based on multilocus enzyme electrophoresis (MLEE), L. infantum MON-1 is the predominant zymodeme in these countries, while the genetically close L. infantum MON-98 zymodeme is occasionally reported in Cyprus, Egypt, Greece, Portugal and Turkey. Nowadays, the distribution range of CanVL has surpassed the limits of South Europe and is spreading toward non-endemic northern European countries. Concurrently, considerable increase in VL cases is documented in traditionally endemic South European countries. However, the epidemiology of VL due to L. infantum spp. in the Balkans and Southeastern (SE) Europe has been either inadequately or never examined so far. In the current study, L. infantum strains (human and canine isolates) were collected from six SE European countries (Albania, Bulgaria, Croatia, Cyprus, Greece and Turkey) and characterized at species and subspecies level by K26-PCR and MLEE. These L. infantum MON-1 and MON-98 strains were then subjected to microsatellite typing (MLMT) using 14 polymorphic loci and compared to previously analyzed L. infantum MON-1 strains from Southwestern (SW) European countries (France, Portugal and Spain) aiming to evaluate genetic diversity and infer the population structure. Our findings confirm the predominance of zymodeme MON-1 in Albania, Bulgaria and Croatia while a considerable number of strains from Greece and Turkey were characterized as L. infantum MON-98. We also demonstrate the existence of geographically-determined populations between SW and SE Europe and successive substructuring among L. infantum strains from Albania, Bulgaria, Croatia, Greece and Turkey, which indicates genetic differentiation, gene flow and possible recombination events. In contrast, our study revealed genetic isolation and clonal reproduction for MON-1 strains from canines in Cyprus. This is the first genetic study that gives insight into the population structure of L. infantum in SE Europe, providing valuable insights into the dynamics of VL in these countries.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA