Naslov
Screening of bacterial lipid libraries by MALDI-TOF PSD and MALDI-QTOF CID

Autori
Beckedorf, Anke I. ; Jovanović, Marko ; Fischer, Werner ; Schaeffer, Christina ; Messner, Paul ; Peter-Katalinić, Jasna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of the 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, Florida, June 2-6, 2002 / - , 2002

Skup
50th ASMS Conference on Mass Spectrometry and Allied Topics

Mjesto i datum
Orlando (FL), Sjedinjene Američke Države, 02.06.2002. - 06.06.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
lipids; MALDI MS; Q-TOF CID; PSD

Sažetak
Bacterial lipid libraries exhibit a high degree of structural variety in context of substitution patterns of fatty acids as well as structural features of the non-lipid part of the molecule. Cardiolipins are tetraacylphospholipids, which are components of the inner mitochondrial membrane and the cytoplasmic membrane. They can be substituted by glucose, alanine or lysine as has already been detected and identified by FAB-MS (1-4) and negative ion-ESI-MS and - MS/MS (2-6). Cardiolipins from Geobacillus stearothermophilus NRS 20004/3a contain fatty acids of different chain length, as already shown by us using ESI-QTOF-MS, CID and negative ion parent ion scanning (7). Here we report on a novel strategy for screening of bacterial lipid libraries by positive and negative ion MALDI-TOF PSD and MALDI-QTOF-CID.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biotehnologija

POVEZANOST RADA