Naslov
Bioinformatics-assisted survey of branching patterns in complex oligosaccharide mixtures

Autori
Jovanović, Marko ; Ocelić, Nenad ; Peter-Katalinić, Jasna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of the 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, Florida, June 2-6, 2002 / - , 2002

Skup
50th ASMS Conference on Mass Spectrometry and Allied Topics

Mjesto i datum
Orlando (FL), Sjedinjene Američke Države, 02.06.2002. - 06.06.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
oligosaccharides; bioinformatics; Q-TOF CID

Sažetak
Recently, MALDI has been coupled to Q-TOF and TOF-TOF mass analyzers, enabling high throughput sequencing of complex carbohydrate mixtures. However, unlike for proteomics, these instruments do not offer any oligosaccharide-specific related software tools, which makes small scale analysis timeconsuming, and high throughput all but impossible. Here we show how use of modern scripting languages like Python and Ruby can be used for writing simple algorithms for various types of oligosaccharide MS and MS/MS data analysis, thus increasing the speed of data interpretation 10 to 100 fold and enabling high-throughput analysis of glycoconjugates. Human milk oligosaccharides represent classic standards for study of carbohydrate branching patterns, challenged by their branching complexity and fucosylation patterns. Introducing bioinformatics-assisted approach for signal assignment and diagnostic ion scanning enables a complete, detailed analysis of such complex biochemical mixtures is feasible.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biotehnologija

POVEZANOST RADA