Naslov
Fragmentation of native and peracetylated oligosaccharides in PSD MALDI-TOF and CID MALDI Q-TOF MS

Autori
Jovanović, Marko ; Willetts, Matthew ; Tyldesley, Richard ; Brown, Jeff ; Peter-Katalinić, Jasna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of the 49th ASMS Conference on Mass Spectrometry and Allied Topics, Chicago, Illinois, May 27-31, 2001 / - , 2001

Skup
49th ASMS Conference on Mass Spectrometry and Allied Topics

Mjesto i datum
Chicago (IL), Sjedinjene Američke Države, 27.05.2001. - 31.05.2001

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
MALDI Q-TOF; CID; oligosaccharides

Sažetak
Glycosylation, ubiquitous to both eukaryotic and prokaryotic proteins, may play an eminent role in modulation of protein function. Glycans modulate function of proteins as determinants of the membrane surface structures, interact with growth factor receptors and extracellular matrix proteins, and serve as cellular mediators for toxins and agonists. New demands for structure elucidation of complex carbohydrates arose from the functional genomics, in-gel deglycosylation and sequencing of glycoforms from complex mixtures. Here we present a new instrumental approach to sequence components of native and derivatized complex oligosaccharide mixtures from biological sources by CID-fragmentation on the new type of instrument, a MALDI Q-TOF and compare to a PSD MALDI TOF approach.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biotehnologija

POVEZANOST RADA