Pregled bibliografske jedinice broj: 656734
Identification of DNA-PKcs phosphorylation sites in XRCC4 and effects of mutations at these sites on DNA end joining in a cell-free system
Identification of DNA-PKcs phosphorylation sites in XRCC4 and effects of mutations at these sites on DNA end joining in a cell-free system // DNA repair, 3 (2004), 3; 267-276 doi:10.1016/j.dnarep.2003.11.005 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 656734 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Identification of DNA-PKcs phosphorylation sites in XRCC4 and effects of mutations at these sites on DNA end joining in a cell-free system
Autori
Kyung-Jong, Lee ; Jovanović, Marko ; Udayakumar, Durga ; Bladen, Catherine L. ; Dynan, William S.
Izvornik
DNA repair (1568-7864) 3
(2004), 3;
267-276
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
XRCC4; DNA-PKcs; phosphorylation; NHEJ; double-strand breaks
Sažetak
Nonhomologous end joining (NHEJ) is the principal mechanism for repairingDNAdouble-strand breaks in mammalian cells. NHEJ requires at least three protein components: theDNA-dependent protein kinase catalytic subunit(DNA-PKcs), Ku protein, and theDNAligaseIV/XRCC4 (DNL IV/XRCC4) complex. Although DNA-PKcs phosphorylates several sites within itself and these other proteins, the significance of phosphorylation at individual sites is not yet understood. Here we investigate the effects of DNA-PKcs-mediated phosphorylation at two sites in XRCC4. One is a previously described site at serine 260 ; the other is a newly mapped site at serine 318. XRCC4 bearing mutations at these sites was co-expressed with DNL IV, the resulting complexes were purified, and activity was tested in a cell-free end-joining system reconstituted from recombinant and purified proteins. Substitution of alanine for serine 260 or 318, which prevents phosphorylation at these positions, or aspartate for serine 260, which mimics constitutive phosphorylation, had no significant effect on overall end-joining activity. In the assay system used, DNA-PKcs is not essential, but when present, arrests the reaction until phosphorylation occurs, in effect establishing a reaction checkpoint. Mutations at serines 260 and 318 did not affect establishment or release from the checkpoint. Results demonstrate that DNA-PKcs-mediated phosphorylation of XRCC4 serine 260 and serine 318 does not directly control end-joining under the conditions tested.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Biotehnologija
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Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
