Naslov
Impact of fumonisin B1 on glutamate toxicity and low-magnesium induced seizure activity in neuronal primary culture

Autori
Domijan, Ana-Marija ; Kovač, Stjepana ; Abramov, Y. Andrey

Izvornik
Neuroscience (0306-4522) 202 (2012); 10-16

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
mycotoxins; epilepsy seizure; mitochondria; calcium signal; acute effects; fumonisin B1

Sažetak
Fumonisin B1 (FB1) is a mycotoxin produced by Fusarium spp. mould that contaminates maize world-wide. Although its neurodegenerative potential is well established, mechanisms and acute effects of FB1 on neurons are still not completely understood. Our previous study on astrocytes and neuroblastoma cells demonstrated that acute FB1 exposure inhibits mitochondrial complex I and leads to mitochondrial membrane potential depolarization and calcium deregulation. To further explore the mechanisms of FB1 neurotoxicity, we here investigated the effects of acute FB1 co-exposure with glutamate and in the low magnesium model of epilepsy on neuronal calcium level, mitochondrial membrane potential, and cell death in glio-neuronal cultures. FB1 increased the glutamate-induced calcium signal in neurons and changed neuronal calcium signals to more sustained intracellular calcium rises in the low magnesium model of epilepsy that coincided with mitochondrial membrane potential depolarization. FB1 co-exposure increased the percentage of dead neurons in low magnesium conditions dose dependently when compared with low magnesium exposure only, whereas in FB1 and glutamate co-exposure neuronal death remained unchanged when compared with glutamate treatment only. Our results show that FB1 makes neurons more vulnerable to glutamate-induced toxicity and epileptiform conditions, indicating that FB1 can enhance the detrimental effect of these conditions on neurons.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Javno zdravstvo i zdravstvena zaštita

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