Pregled bibliografske jedinice broj: 65579
DNA and echinochrome production in sea urchin as biochemical indicators of tributyltin toxicity
DNA and echinochrome production in sea urchin as biochemical indicators of tributyltin toxicity // 6. EMINar : Book of Abstracts / Bakran-Petricioli, Tatjana ; Juračić, Mladen ; Absil (ur.).
Zagreb, 1997. str. 60-60 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 65579 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
DNA and echinochrome production in sea urchin as biochemical indicators of tributyltin toxicity
Autori
Petrović, Siniša ; Ozretić, Bartolo ; Krajnović-Ozretić, Mirjana
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
6. EMINar : Book of Abstracts
/ Bakran-Petricioli, Tatjana ; Juračić, Mladen ; Absil - Zagreb, 1997, 60-60
Skup
EMINar (6 : 199/)
Mjesto i datum
Sali, Hrvatska, 02.04.1997. - 06.04.1997
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
DNA; echinochrome; sea urchin; tributyltin
Sažetak
Tributyltin (TBT) is a highly toxic synthetic compound used as additive to the antifouling submarine paints to prevent the attachment of larvae and their growth on the submerged surface of ship hulls. The antifouling prevention is based on the continuous leaking of TBT in the sea water roundabout the ship hulls acting as a toxic barrier to the fouling organisms. On the other side, because of its highly toxic and genotoxic properties, TBT generates serious harm to the marine environment in general but particularly to the larval and juvenile stages of marine invertebrates from the bottom communities. The toxicity of TBT was evaluated using sea urchin, Paracentrotus lividus Lam., gametes and their early developmental stages as recommended model organism. Various concentrations of TBT were obtained by leakage from painted plates or by addition of pure TBTO in sea water. The toxicity of TBT was estimated measuring the total DNA and echinochrome production after 5 and 30 h of embryo development. Both parameters are non-specific biochemical indicators of physiological activity and growth rate. These tests were compared with some morphological procedures for the TBT toxicity assesment. Among them, fertilization membrane formation, cleavage rate, hatching of blastulae and appearing of various morphological deformations were estimated. However, the most accurate quantitative results were obtained by the measurement of DNA and echinochrome production. The swimming blastulae appeared to be the most sensitive stage and the measurement of their DNA content can detect the toxic effects of TBT inside few hours.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
