Naslov
Sequencing of the Ephydatia muelleri genome: preliminary results

Autori
Maša Roller, Mirna Imešek, Vedran Franke, Matija Harcet, Gert Wörheide, April Hill, Helena Ćetković, Sally Leys and Kristian Vlahoviček

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Ninth World Sponge Conference 2013

Mjesto i datum
Fremantle, Australija, 04.11.2013. - 08.11.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
muelleri; genomics; genome assembly; COG; next-generation sequencing

Sažetak
With their unique evolutionary position of arguably the simplest extant and earliest branching metazoans, sponges are rapidly gaining focus in genomic research. Only one draft genome sequence of a sponge has been published to date, that of Amphimedon queenslandica. Genomic information from additional taxa is required for comparative genomic approaches within the phylum Porifera to describe their genomic makeup, gene architecture and structure, eventually leading to elucidation of early metazoan gene regulation and development. We sequenced and assembled a preliminary draft of the genome of the freshwater sponge Ephydatia muelleri using next-generation sequencing technology. Paired- end Illumina libraries yielded contigs covering at least 85% of the genome. Addition of SOLiD mate-pair libraries produced scaffolds covering the entire genome. We identified a preliminary gene set through automated annotation and functionally classified them using the STRING Clusters of Orthologous Groups (COG) database. Further sequencing is currently underway to improve coverage and complete the genome.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA