Naslov
Optimal immunohaematological method-eternal enigma

Autori
Kruhonja Galić, Zrinka ; Hundrić Hašpl, Željka ; Juraković Lončar, Nina ; Tomičić, Maja ; Balija, Melita ; Jukić, Irena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of The XXXIst International Congress of the International Society of Blood Transfusion in joint cooperationwith the 43rd Congress of the DGTI ; u: Vox sanguinis 99 (2010)(S1) 1-541 ; Poster section 91-516, P-0676 / Mayr, W.R. - Oxford : Blackwell Publishing, 2010, 341-341

Skup
International Congress of the International Society of Blood Transfusion (31 ; 2010) ; Congress of the DGTI (43 ; 2013)

Mjesto i datum
Berlin, Njemačka, 26.06.2010. - 01.07.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
optimal immunohaematological method

Sažetak
For more then 50 years, the search is still going on – how to detect all clinically significant erythrocyte (RBC) antibodies (Ab) and to avoid detection of Ab of little or no clinical significance at all. There are few highly sensitive micromethods, but with intention of increasing sensitivity they sometimes decrease in specificity. Our aim was to confirm and to identify RBC Ab by microtube column agglutination techniques in samples which showed visually positive results in the Solid-phase RBC adherence test (SPRCA). We use SPRCA (Solidscreen II, Biotest) in semiautomatic manner on Tecan-Genesis. After Medusa software interpretation, the results were rechecked by experienced laboratory staff visually. We collected 1059 samples which had visual positivity only. With these samples two microtube column agglutination methods (Dia- Med-ID, Ortho BioVue) for antibody screening were performed. RBC Ab identifications of samples with positive results by same techniques were done. Seven hundred and twenty-nine samples (69%) were IAT negative (false positive results). 137(13%) were IAT positive (unwanted RBC Ab) and 193(18%) were IAT positive (wanted RBC Ab). Among 137 samples with IAT positive results due to unwanted RBC Ab 10(7%) was due to the presence of RBC autoAb, 24(18%) of cold RBC Ab, 12(9%) of weak nonspecific positivity and 91(66%) due to the administration of anti-D immunoglobulin. From 193 samples with wanted IAT positive results there were 168(87%) samples with one RBC Ab and 25(13%) with multiple RBC Ab. We found entirely 218 wanted RBC Ab: 99(45%) clinically significant and 119(55%) RBC Ab of minor clinical significance. In Rh blood group system we detected 72 RBC Ab (38 anti-D, 17 anti-E, 10 anti-C, 3 anti-c, 1 anti-e, 1 anti-G, 2 anti-Cw) and 27 in other blood group systems (18 anti-K, 5 anti-Jka, 2 anti-Fya and 2 anti-S). In addition, we found subsequent RBC Ab of minor clinical significance: 60 anti-M, 1 anti-M like, 1 anti-N, 31 anti-Lea, 19 anti-Leb, 5 anti-P1, 1 anti-Lub and 1 anti-Jra. Among 78 samples with SPRCA visually weak positive results only, 48 samples were confirmed IAT positive in the Ortho BioVue only (2 anti-D, 1 anti-C, 1 anti- E, 4 anti-K, 1 Jka, 6 anti-M, 5 anti-Lea, 2 anti-Leb, 2 anti-P1, 1 autoAb, 13 cold RBC Ab, five weak non-specific positivity and five administration of anti-D immoglobulin. In 16 samples equal results in both micromethods, and in 14 samples in DiaMed-ID weaker reactions then in Ortho BioVue were observed. Different immunohaematological methods may be equal, more or less successful in identification of RBC Ab of the same specificity, which depends on characteristics and/or quantity of RBC Ab. The selection of routine test method should be balanced between sensitivity, specificity and cost benefit of test method. Because of that, the choice of optimal immunohaematological method was, still is and will be eternal enigma.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

Napomena
DOI: 10.1111/j.1423-0410.2010.01343_2.x

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