Naslov
A direct ion-selective electrode analyzer (NOVA 10)evaluated for serum Li determination

Autori
Ferenec-Ružić, Dragica ; Blazinić, Franciska ; Vrkić, Nada ; Antoljak, Nataša ; Lazić, N

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Biochemia Medica, vol 10, 6th Alps-Adria congress of clinical chemistry and laboratory medicine- abstracts / Suchanek, Ernest - Zagreb : Hrvatsko društvo za medicinsku biokemiju i laboratorijsku medicinu (HDMBLM), 2000

Skup
6th Alps-Adria congress of clinical chemistry and laboratory medicine

Mjesto i datum
Opatija, Hrvatska, 15.06.2000. - 17.06.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
ISE analyzer; NOVA 10; Li

Sažetak
A ion-selective electrode analyzers for measuring lithium (Li/ISE) in serum became available in early 1987. Since that time, several ionophores for determination of lithium in serum have been described. NOVA 10 (NOVA Biomedical, Waltham, MA) is a direct, ion-selective electrode analyzer for measuring sodium, potassium, chloride, total calcium and lithium in serum, plasma, CSF and urine. The aim was to evaluate the analytical performance of the instrument according to the ECCLS guidelines (imprecision, accuracy, carryover, linearity and comparison study for lithium (Li) in serum. Venous blood from patients receiving lithium therapy was collected in to vacutainer tubes (Becton Dickinson Co, Rutherford, UK) with no anticoagulant, and allowed to clot. Serum was separated by centrifugation. Control material used in the study included Stat Profile Control 1-3, Precinorm U, Precipath U and Serodos. Randomly chosen patient sera were used in comparison study for lithium on a flame photometer PHF-104 (Hycel, France). Intra-assay CV was 1.08%. While interassay CV ranged from 1.05% to 1.15%. Carryover was negligible (K<0, 01%). Linearity was within the declared analytical range. Results of the comparison study (Passing Bablok) showed no statistically significant difference compared with flame photometer (y=0.90x+0.00 ; r=0, 9939). In conclusion, NOVA 10 is a precise and accurate instrument, and can be used as a reliable method for measuring Li concentrations in serum samples.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija

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