Naslov
Hepatic expression of metallothionein I/II, glycoprotein 96, IL-6, and TGF-beta in rat strains with different susceptibility to experimental autoimmune encephalomyelitis

Autori
Grubić-Kezele, Tanja ; Blagojević Zagorac, Gordana ; Jakovac, Hrvoje ; Domitrović, Robert ; Milin, Čedomila ; Radošević-Stašić, Biserka

Izvornik
Clinical & developmental immunology (1740-2522) (2013); 750406-1

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
experimental autoimmune encephalomyelitis; liver; metallothionein I/II; glycoprotein 96; IL.6; TGF beta

Sažetak
In a search of peripheral factors that could be responsible for the discrepancy in susceptibility to EAE in Albino Oxford (AO) and Dark Agouti (DA) rats, we estimated the expression of metallothioneins I/II (MT), heat shock protein-gp96, interleukin (IL)-6 and transforming growth factor (TGF)-beta in the livers of these animals. Rats were immunized with bovine brain homogenate (BBH) emulsified in complete Freund adjuvant (CFA) or only with CFA. Western blot and immunohistochemical analyses were done on day 12 after the immunization, as well as in intact rats. The data have shown that during the first attack of EAE only the EAE prone-DA rats markedly upregulated the hepatic MTs, gp96, IL-6 and TGF- beta. In contrast, AO rats had a significantly higher expression of MT I/II, IL-6 and TGF-beta in intact liver (p<0, 001), suggesting that the greater constitutive expression of these proteins contributed to the resistance of EAE. Besides, since previously we found that AO rats reacted on immunization by an early upregulation of TGF-beta on several hepatic structures (vascular endothelium, Kupffer cells and hepatocytes), the data suggest that the specific hepatic microenvironment might contribute also to the faster recovery of these rats from EAE

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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