Pregled bibliografske jedinice broj: 65231
The mitotic activity and the distribution of nuclei in vegetative buds of Norway spruce (Picea abies) in vitro and in vivo
The mitotic activity and the distribution of nuclei in vegetative buds of Norway spruce (Picea abies) in vitro and in vivo // Proceedings of the First IUFRO Cytogenetics Working Party 2.04-08 Symposium. Cytogenetics studies of forest trees and shrub species / Borzan, Želimir ; Schlarbaum, Scott.E. (ur.).
Zagreb: Hrvatske šume, 1997. str. 121-130 (predavanje, međunarodna recenzija, cjeloviti rad (in extenso), znanstveni)
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Naslov
The mitotic activity and the distribution of nuclei in vegetative buds of Norway spruce (Picea abies) in vitro and in vivo
Autori
Cesar, Vera
Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni
Izvornik
Proceedings of the First IUFRO Cytogenetics Working Party 2.04-08 Symposium. Cytogenetics studies of forest trees and shrub species
/ Borzan, Želimir ; Schlarbaum, Scott.E. - Zagreb : Hrvatske šume, 1997, 121-130
Skup
First IUFRO Cytogenetics Working Party S2.04-08 Symposium
Mjesto i datum
Brijuni, Hrvatska, 08.09.1993. - 11.09.1993
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
conifers; Picea abies (L.) Karst; vegetative buds; mitotic activity; tissue culture
(conifers; Picea abies (L.) Karst.; vegetative buds; mitotic activity; tissue culture)
Sažetak
The mitotic activity of vegetative buds of Picea abies grown in vitro was compared with mitotic activity of buds grown in nature. The distribution of nuclei and the mitotic index (Mi) in the embryonic shoot was determined. Longitudinal 3 um-thin sections of fixed vegeteative buds of Picea abies from in vitro and in vivo conditions were successively cut from the beginning to the middle of the bud and examined. During winter, the cells had large, round nuclei, and no cell divisions were observed. The first cell divisions were observed with onset of spring. In two weeks, the mitotic index had increased from 3.6x10-3 to 44.1x10-3. The divisions were periclinal in the dermal layer of cells in the apex. In needle primordia, mitotic spindles were orientated in the way of primordia elongation with few anticlinal mitoses in mid-cortex. The mitotic index in the buds grown in vitro was 3.3x10-3 to 2.9x10-3. In the regions with induced adventitious buds, the divisions were anticlinal in the dermal layer of cells and anticlinal and periclinal in the layers below. In isolated adventitious buds, the mitotic index was 5.8x10-3.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
