Naslov
Distribution of complex gangliosides in brains of catfish and perch

Autori
Viljetić, Barbara ; Labak, Irena ; Blažetić, Senka ; Ivić, Vedrana ; Heffer, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts 4th Croatian Congress of Neuroscience / / - Zagreb : Hrvatsko društvo za neuroznanost ; Hrvatski institut za istraživanje mozga Medicinskog fakulteta Sveučilišta u Zagrebu, 2013, 34-34

Skup
4th Croatian Congress of Neuroscience

Mjesto i datum
Zagreb, Hrvatska, 20.09.2013. - 21.09.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Gangliosides; catfish; perch; TLC; immunohistochemistry
(Gangliozidi; som; smuđ; TLC; imunohistokemija)

Sažetak
Gangliosides are acidic glycosphingolipids containing one or more sialic acid. In contrast to higher vertebrates where mono-, di- and trisialo gangliosides dominate, fish brain is abundant in polisialo structures with important role in adaptations to environmental conditions. Until recently the distribution of gangliosides was usually studiet in rodent and mammalian brains. The aim of this study was to reveal distribution of the main gangliosides in the brains of two species of the Actinopterygian subclass: catfish (Silurus gladis) and perch (Perca fluviatilis). Gangliosides were extracted from adult brains of catfish and perch and separated by TLC. High affinity anti-ganglioside (GM1, GD1a, GD1b, GT1b)IgG antibodies were used for immunohistochemistry. Contrary to higher vertebrates with uniform pattern of brain ganglioside expression, investigated species varied in ganglioside distribution. Gangliosides GD1b predominates in catfish brain from telencephalon to the spinal cord. In perch brain all four major gangliosides were detected. GM1 and GT1b are expressed on neurons and not on fibers like in mammalians ; GD1b was detected in all structures of perch brain. Both studied species had a different expression of complex gangliosides suggesting that characteristic distribution is dual to adaptations to different lifestyles and habitats.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA