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Pregled bibliografske jedinice broj: 650939

Two fluorogenic substrates for purine-nucleoside phosphorylase, selective for mammalian and bacterial forms of the enzyme

Wierzchowski, Jacek; Stachelska-Wierzchowska, Alicja; Wielgus-Kutrowska, Beata; Mikleušević, Goran
Two fluorogenic substrates for purine-nucleoside phosphorylase, selective for mammalian and bacterial forms of the enzyme // Analytical biochemistry, 446 (2014), 25-27 doi:10.1016/j.ab.2013.10.017 (podatak o recenziji nije dostupan, vijest, znanstveni)

CROSBI ID: 650939 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Two fluorogenic substrates for purine-nucleoside phosphorylase, selective for mammalian and bacterial forms of the enzyme

Autori
Wierzchowski, Jacek ; Stachelska-Wierzchowska, Alicja ; Wielgus-Kutrowska, Beata ; Mikleušević, Goran

Izvornik
Analytical biochemistry (0003-2697) 446 (2014); 25-27

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, vijest, znanstveni

Ključne riječi
fluorogenic substrates; 8-azapurines; nucleoside analogs; purine-nucleoside phosphorylase

Sažetak
Two non-typical nucleosides, 7-β-D-ribosyl-2, 6-diamino-8-azapurine and 8-β-D-ribosyl-2, 6-diamino-8-azapurine, have been found to exhibit moderately good, and selective, substrate properties towards calf and bacterial (E. coli) forms of purine-nucleoside phosphorylase (PNP). The former compound is effectively phosphorolysed by calf PNP, and the latter by PNP from E. coli. Both compounds are fluorescent with λmax ∼ 425-430 nm, but the reaction product, 2, 6-diamino-8-azapurine, emits in different spectral region (λmax ∼ 363 nm), with nearly 40% yield, providing strong fluorogenic effect at 350-360 nm.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA

Projekti:
098-1191344-2943 - Protein-ligand međudjelovanja na atomnoj razini (Luić, Marija, MZOS ) ( CroRIS)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Goran Mikleušević (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com

Citiraj ovu publikaciju:

Wierzchowski, Jacek; Stachelska-Wierzchowska, Alicja; Wielgus-Kutrowska, Beata; Mikleušević, Goran
Two fluorogenic substrates for purine-nucleoside phosphorylase, selective for mammalian and bacterial forms of the enzyme // Analytical biochemistry, 446 (2014), 25-27 doi:10.1016/j.ab.2013.10.017 (podatak o recenziji nije dostupan, vijest, znanstveni)
Wierzchowski, J., Stachelska-Wierzchowska, A., Wielgus-Kutrowska, B. & Mikleušević, G. (2014) Two fluorogenic substrates for purine-nucleoside phosphorylase, selective for mammalian and bacterial forms of the enzyme. Analytical biochemistry, 446, 25-27 doi:10.1016/j.ab.2013.10.017.
@article{article, author = {Wierzchowski, Jacek and Stachelska-Wierzchowska, Alicja and Wielgus-Kutrowska, Beata and Mikleu\v{s}evi\'{c}, Goran}, year = {2014}, pages = {25-27}, DOI = {10.1016/j.ab.2013.10.017}, keywords = {fluorogenic substrates, 8-azapurines, nucleoside analogs, purine-nucleoside phosphorylase}, journal = {Analytical biochemistry}, doi = {10.1016/j.ab.2013.10.017}, volume = {446}, issn = {0003-2697}, title = {Two fluorogenic substrates for purine-nucleoside phosphorylase, selective for mammalian and bacterial forms of the enzyme}, keyword = {fluorogenic substrates, 8-azapurines, nucleoside analogs, purine-nucleoside phosphorylase} }
@article{article, author = {Wierzchowski, Jacek and Stachelska-Wierzchowska, Alicja and Wielgus-Kutrowska, Beata and Mikleu\v{s}evi\'{c}, Goran}, year = {2014}, pages = {25-27}, DOI = {10.1016/j.ab.2013.10.017}, keywords = {fluorogenic substrates, 8-azapurines, nucleoside analogs, purine-nucleoside phosphorylase}, journal = {Analytical biochemistry}, doi = {10.1016/j.ab.2013.10.017}, volume = {446}, issn = {0003-2697}, title = {Two fluorogenic substrates for purine-nucleoside phosphorylase, selective for mammalian and bacterial forms of the enzyme}, keyword = {fluorogenic substrates, 8-azapurines, nucleoside analogs, purine-nucleoside phosphorylase} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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