Naslov
AEROMONAS SALMONICIDA INFECTIONS IN SALMONIDS

Autori
Kapetanović, Damir ; Vardić Smrzlić, Irena ; Valić, Damir ; Muharemović, Hasan ; Teskeredžić, Emin

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Days of Veterinary Medicine 2013 / Mitrov, Dine ; Pendovski, Layo ; Percinic, Florina P. - Skopje : Faculty of Veterinary Medicine - Skopje, 2013, 89-90

ISBN
978-9989-774-25-6

Skup
4th International Scientific Meeting Days of Veterinary Medicine 2013

Mjesto i datum
Struga, Sjeverna Makedonija, 06.09.2013. - 08.09.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Aeromonas salmonicida; Arctic charr; brown trout; MALDI TOF; PCR

Sažetak
Management of furunculosis remains a considerable clinical challenge, in part due to their ability to cause disease in the different salmonids, both in the culture systems and in the wild environment. Aeromonas salmonicida subsp. salmonicida is a rapidly spreading pathogen associated predominantly with skin infections in the salmonids with high mortality and the disease can cause major economical losses in the hatcheries. These characteristics provide a challenge to clinicians for the fast and successful diagnosis and therapy. Here we report the isolation of A. salmonicida from different salmonids and evaluating identification methods and antimicrobial sensitivity of these isolates. A. salmonicida isolates were isolated and characterized based on their morphology and host range, and evaluated for their antimicrobial sensitivity. Isolates were from Arctic charr (Salvelinus alpinus) and brown trout (Salmo trutta) obtained on the Tryptic Soy Agar plates (BD-BBL) after incubation for 24-48h at 22ºC. Phenotypical characteristics, Gram staining and oxidase production were determined for isolates and further characterization was carried out by using the API kits (Bio Mérieux). Isolates were also analyzed using MALDI TOF (Bruker) mass spestrometry with confirmation of isolated bacteria by PCR. The antimicrobial susceptibility of the isolated strains was determined with Kirby-Bauer disk diffusion method on Difco™ Mueller Hinton II agar (Becton, Dickinson and Company) using the discs obtained from the same manufacturer. The following antimicrobial discs were used in antibiogram: ampicillin (AM10), chloramphenicol (C30), enrofloxacin (ENO5) erythromycin (E15), flumequine (AR30), nitrofurantion (F/M300), norfloxacin (NOR10), novobiocin (NB5), oxytetracycline (T30), penicillin (P10), piperacillin (PIP100), sulfamethoxazole/trimethoprim (SXT), tetracycline (TE30) and trimethoprim (TMP5). Only one type of colony was recovered in pure culture on Tryptic soy agar media from all positive organs from examined fish in open waters and culture systems i.e. brown trout and Arctic charr. Gram staining revealed the presence of gram-negative bacilli. The colony characteristic was brown diffusible pigment on the agar. The identification of bacterial isolates by API tests, MALDI TOF mass spestrometry and PCR analysis confirmed the presence of A. salmonicida. Resistance was recorded for ampicillin, enrofloxacin, erythromycin, norfloxacin, novobiocin, penicillin, piperacillin, tetracycline and trimethoprim The results obtained in this study suggest that A. salmonicida exist in the fish from open waters as well as in the culture systems of salmonids. That fact might increase the possibilities of transfer of this pathogenic bacterium.

Izvorni jezik
Engleski

Znanstvena područja
Geologija, Biologija, Veterinarska medicina

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