Naslov
Detection of DNA damage in haemocytes of zebra mussel using comet assay

Autori
Pavlica, Mirjana ; Klobučar, Goran Igor Vinko ; Mojaš, Nina ; Erben, Radovan ; Papeš, Dražena

Izvornik
Mutation Research (0027-5107) 490 (2001); 209-214

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Dreissena polymorpha; zebra mussel; haemocytes; comet assay; PCP; biomonitoring

Sažetak
The aim of the study was to use the comet assay on haemocytes of freshwater mussel, Dreissena polymorpha Pallas, for detection of possible DNA damage after exposure to pentachlorophenol (PCP) and to evaluate the potential application of the comet assay on mussel haemocytes for genotoxicity monitoring of freshwater environment. Zebra mussels were exposed for seven days to different concentrations (10, 80, 100, 150  g/l) of PCP and in the river Sava downstream from Zagreb municipal wastewater outlet. Significant increase in DNA damage was observed after exposure to PCP at doses of 80  g/l and higher and after in situ exposure in the river Sava as well. This study confirmed that the comet assay applied on zebra mussel haemocytes may be a useful tool in determining the potential genotoxicity of water pollutants.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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