Pregled bibliografske jedinice broj: 64689
Methionine Adenosyltransferase Purified From Rat Liver
Methionine Adenosyltransferase Purified From Rat Liver // Croatica chemica acta, 75 (2002), 1; 247-254 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 64689 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Methionine Adenosyltransferase Purified From Rat Liver
Autori
Pérez-Mato, Isabel ; Matak-Vinković, Dijana ; Vinković, Mladen ; Mato ; José M.
Izvornik
Croatica chemica acta (0011-1643) 75
(2002), 1;
247-254
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
methionine adenosyltransferase; enzyme; microcrystals; microdiffraction
Sažetak
Methionine adenosyltransferase (MAT III), also known as S-adenosylmethionine synthetase, was purified from rat liver and crystallized. X-ray diffraction data were collected using a microfocused synchrotron radiation. The crystallization conditions were extensively optimized but final crystal size was never larger than 303 mm3. Due to their small size crystals had no detectable diffraction on either rotating anode source or the Deresbury SRS beamline 9.6 (GB). Finally, four data sets were collected on Grenoble ESRF (France) undulator microfocus beamline ID13 to resolution of 3.2-3.6 A. Crystals belong to the cubic space group F432 with cell dimension a = 246 A. Attempts are under way to solve the structure by molecular replacement, using recombinant MAT I rat liver structure as a search model.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- SCI-EXP, SSCI i/ili A&HCI
