Naslov
Immunophenotyping of lesions in atopic dermatitis patients

Autori
Lipozenčić, Jasna, Lugović, Liborija ; Jakić-Razumović, Jasminka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Journal of the European Academy of Dermatology and Venereology (Abstracts) / - : Wiley-Blackwell, 2000

Skup
9th Congress of the European Academy of Dermatology and Venereology

Mjesto i datum
Ženeva, Švicarska, 11.10.2000. - 15.10.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Immunophenotyping in atopic dermatitis patients; immunohistochemistry in dermatology

Sažetak
Immunohistochemistry analysis was performed in a group of atopic (AD) patients. Cell immunophenotyping was carried out in the biopsies of skin lesions from 15 AD patients and in 5 healthy skin biopsies as control. The method of avidin-biotin immunoperoxydase on paraffin 4um skin sections and semy quantitative counting of cells marked with anti-CD20, anti-CD8, anti-CD4 , anti-HLA-DR and anti-IgE primar antibodies were performed. Results of AD skin analysis showed a greater infiltration of CD3+ lymphocytes, especially of CD4+ subtype compared with CD8+ lymphocytes. AD skin biopsies showed a higher expression of HLA-DR+ Langerhans' cells in the epidermis, presence of HLA-DR on lymphocytes in the dermis , and higher expression of IgE+ cells in comparison to healthy control. A statistically significant difference(p <0.05) was found among the two groups in CD3, CD4, HLA-DR in dermis and epidermis, CD8 in dermis, IgE+ cells in epidermis.Our results confirm that immunophenotyping detect significant alterations in AD patients.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA