Pregled bibliografske jedinice broj: 645496
The YEATS domain protein TAF14 exhibits complex regulatory properties across the genome
The YEATS domain protein TAF14 exhibits complex regulatory properties across the genome // Mechanisms of eukaryotic transcription / Buratowski, Stephen ; Jones Katherine ; Lis John (ur.).
Lahti, 2013. (poster, nije recenziran, sažetak, znanstveni)
CROSBI ID: 645496 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The YEATS domain protein TAF14 exhibits complex regulatory properties across the genome
Autori
Nemet, Josipa ; Jeličić, Branka ; Sopta Marija-Mary
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Mechanisms of eukaryotic transcription
/ Buratowski, Stephen ; Jones Katherine ; Lis John - Lahti, 2013
Skup
Mechanisms of eukaryotic transcription
Mjesto i datum
Cold Spring Harbor (NY), Sjedinjene Američke Države, 27.08.2013. - 31.08.2013
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
Taf14 protein; transcription regulation; genome wide
Sažetak
Taf14, a Saccharomyces cerevisiae protein, physically associates with many critical multisubunit complexes including the general transcription factors TFIID and TFIIF, and the chromatin modifying complexes SWI/SNF, INO80, RSC and NuA3. Taf14 is also a member of the highly conserved YEATS domain superfamily of proteins. Given that little is known about the role of Taf14 in transcription and chromatin remodeling we investigated the impact of TAF14 deletion on genome wide transcription in yeast. Using published microarray data we performed a bioinformatic analysis and found that TATA- containing, SAGA-dependent genes were much more affected by TAF14 deletion than were TFIID- dependent genes. We show that Taf14 repressed genes tend to be extensively regulated, positively by SAGA complex and stress-responsive activators and negatively by a wide number of repressors that act upon promoter chromatin and TBP. We further characterized special regulatory features of Taf14 repressed promoters upon transition from normal to acute heat shock conditions. Using data from a previously reported genome-wide binding study for 200 transcription-related proteins, we analysed Taf14 co-occupancy with subunits of previously reported Taf14-containing complexes and discovered distinct sets of subunits that bind in close vicinity to Taf14 on a significant number of analyzed promoters.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
