Naslov
The appearance of eosinophils in nasal mucosa after bronchial allergen chalenge

Autori
Stipić-Marković Asja ; Čvorišćec Branimir ; Ostojić, Vedran ;

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Book of abstracts / NN - Lisabon : European Academy of Allergology and Clinical Immunology, 2000

Skup
Annual meeting of European Academy of Allergology and Clinical Immunology

Mjesto i datum
Lisabon, Portugal, 01.07.2000. - 05.07.2000

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
bronchial challenge; nasal mucosa; allergy; eosinophil

Sažetak
The relationship between inflammatory events in nasal and bronchial mucosa are not entirely known. There is evidence in the literature that inflammatory events in the nose influence responsiveness of the lower respiratory tract. In the present study we explored the opposite relationship - whether allergen challenge of the lower respiratory tract would initiate an inflammatory process in the nose. Forty-two atopic patients, with high levels of specific IgE antibody to house Dust mite and clinical symptoms of allergic disease (32 asthma, 10 perennial rhinitis) were included. Patients underwent bronchial challenge, by Asthma Provocation System, with five-fold increasing concentrations of standardized Dermatophagoides pteronyssinus allergen extract. A gentle scraping-cytocentrifugation method for collection of mucosal specimens was used for nasal cytology. Nasal smears and venous blood were sampled prior to and 2, 6, 12 and 48 hours after allergen stimulation. The results showed that 95% of patients had an early bronchial reaction with a mean fall in PEF of 28ą8%, Peripheral blood eosinophils significantly decreased 6 hours after stimulation (p=0.02) and increased 48 hours after stimulation (p=0.0002). In 20 patients eosinophils appeared in the nasal mucosa. The percentage of eosinophils in nasal smears increased at 2 hours (p=0.008), 6 hours /p=0.02), 12 hours (p=0.015) and 48 hours (p=0.0001) post allergen exposure. Our results indicate that antigen stimulation by means of inhalation through the mouth (nostrils were clipped), in parallel with bronchospasm, induced changes in nasal mucosa without its direct contact with antigen. This cellular mucosal reaction was not accompanied by the clinical expression of nasal symptoms. This phenomenon suggests the existence of a primary immunological abnormality in atopic patients as related to the allergic inflammatory response.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA