Naslov
Comparison of P-glycoprotein activity in gill tissue of two different groups of blue mussel (Mytilus galloprovincialis Lam): aquaculture maintained and polluted environment

Autori
Bošnjak, Ivana ; Mladineo, Ivona ; Hrabar, Jerko ; Šver, Lidija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
11. Hrvatski biološki kongres : zbornik sažetaka / Jelaska, Sven D. ; Klobučar, Goran I.V. ; Šerić Jelaska, Lucija ; Leljak Levanić, Dunja ; Lukša, Žaklin - Zagreb : Hrvatsko biološko društvo, 2012, 261-262

Skup
11. Hrvatski biološki kongres

Mjesto i datum
Šibenik, Hrvatska, 16.09.2012. - 21.09.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
P-glycoprotein (P-gp); xenobiotics; Mytilus galloprovincialis; aquaculture; polluted environment

Sažetak
The success for an organism to adapt to pollution in aquatic environments depends on the efficiency of primary detoxification cell machineries such as ATP-binding cassette (ABC) transporter proteins which act as first line defense and are capable to actively efflux out vast variety of xenobiotics present in water. As mussels are filter feeders our focus was to test transport activity of P- glycoprotein (Pgp ; ABCB-type transporter) in the gill tissue of blue mussels (Mytilus galloprovincialis Lam). We assayed the modulation of Pgp transporter activity by inhibition of substrate transport test using Pgp specific substrate (rhodamine B) and Pgp specific inhibitor (Verapamil). The Pgp transport activity was measured in the gill tissue of aquaculture mussels harvested in pristine sea water (Marina bay, Croatia) and native mussels collected from highly polluted site (Vranjic bay, Croatia) in the presence of 0 µM (control group), 0.01 µM, 0.1 µM and 1 µM inhibitor concentration. Pgp transport activity was measured immediately after 2-hour exposure and in long-term recovery period in clean sea water (24 hours). The results of 2-hour exposure indicate double efficient efflux of Pgp in gill tissue of aquaculture in compare to the native mussels. But 24 hours recovery period resulted in more efficient recovery of Pgp inhibitory effects in gill tissue of native mussels. Our results suggest that native mussels have more adapted ABC transporters that manage them survival in the polluted sea water.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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