Naslov
An immunodominant site of acetylcholine receptor in experimental myasthenia mapped with T lymphocyte clones and synthetic peptides
(An immunodominant site of acetylcholine receptor in experimental myasthenia mapped with T lymphocyte clones and synthetic peptides)

Autori
Pachner, A.R. ; Kantor, F.S. ; Mulac-Jeričević, Biserka ; Atassi, M.Z.

Izvornik
Immunology letters (0165-2478) 20 (1989), 3; 199-204

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
acetylcholine receptor ; myasthenia gravis ; T cell

Sažetak
To study the organization of the polypeptide chain of the main extracellular domain of the nicotinic acetylcholine receptor (AChR) alpha-subunit, we examined the ability of the native membrane-bound AChR of Torpedo californica (T-AChR) to bind a panel of antibodies against overlapping synthetic peptides which collectively encompassed this entire domain. Antibodies against the alpha-chain peptides alpha 1- 16, alpha 89-104 and alpha 158- 174 were able to bind to membrane-bound T-AChR. Other anti-peptide antibodies showed little or no binding to T-AChR in the membrane. It is concluded that regions alpha 1- 16, alpha 89-104 and alpha 158-174 are highly exposed on the surface of the alpha subunit of membrane-bound AChR.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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