Naslov
Immunity Against Heterosubtypic Influenza Virus Induced By Adenovirus And MVA Expressing Nucleoprotein And Matrix Protein-1.

Autori
Lambe, Teresa ; Carey, John B. ; Li, Yuanyuan ; Spencer, Alexandra J. ; van Laarhoven, Arjan ; Mullarkey, Caitlin E. ; Vrdoljak, Anto ; Moore, Anne C. ; Gilbert, Sarah C.

Izvornik
Scientific Reports (2045-2322) 3 (2013); 47-55

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Influenza virus; Viral infection; Mucosal immunology; Vaccines

Sažetak
Alternate prime/boost vaccination regimens employing recombinant replication-deficient adenovirus or MVA, expressing Influenza A virus nucleoprotein and matrix protein 1, induced antigen-specific T cell responses in intradermally (ID) vaccinated mice ; with the strongest responses resulting from Ad/MVA immunization. In BALB/C mice the immunodominant response was shifted from the previously identified immunodominant epitope to a novel epitope when the antigen was derived from A/Panama/2007/1999 rather than A/PR/8. Alternate immunization routes did not affect the magnitude of antigen-specific systemic IFN-γ response, but higher CD8+ T-cell IFN-γ immune responses were seen in the bronchoalveolar lavage following intransal (IN) boosting after intramuscular (IM) priming, whilst higher splenic antigen-specific CD8+ T cell IFN-γ was seen following IM boosting. Partial protection against heterologous influenza virus challenge was achieved following either IM/IM or IM/IN but not ID/ID immunization. These data may be of relevance for the design of optimal immunization regimens for human influenza vaccines, especially for influenza-naïve infants.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Farmacija, Biotehnologija

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