Naslov
Human identification from the gut of blowfly larvae (Calliphoridae)

Autori
Hercog, Rajna ; Ćurić, Goran

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
The 7th ISABS Conference in Forensic, Anthropologic and Medical Genetics and Mayo Clinic Lectures in Translational Medicine: The book of abstracts / Vuk-Pavlović, Stanimir ; Primorac, Dragan ; Schanfield, Moses - Zagreb : International Society for Applied Biological Sciences (ISABS), 2011, 115-115

Skup
The 7th ISABS Conference in Forensic, Anthropologic and Medical Genetics and Mayo Clinic Lectures in Translational Medicine

Mjesto i datum
Bol, Hrvatska, 20.06.2011. - 24.06.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
STR; human DNA; DNA extraction; forensic entomology; fly larvae

Sažetak
Entomological evidence is most commonly used to estimate the postmortem interval, where it is crucial to identify species of insect feeding on the corpse. Analysis of human DNA isolated from insects enables identification of human remains: in cases of identification of corpse displaced from the crime scene, in cases where larvae had alternative food source, or in cases of questionable reliability of collected evidence. The aim of this study was to recover suitable human DNA from the gut of blowfly larvae (Calliphoridae) at different larval stage. Different decontamination, preservation and extraction methods were compared. Obtained DNA profiles were matched to cadaver reference sample, using commercial forensic kits (AmpFlSTR® Identifiler™ and AmpFlSTR® MiniFiler™). The results show that it is possible to obtain human STR profile sufficient for human identification from fly maggot gut at different larval stage, while several technical precautions should be considered.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA