Naslov
The role of S-layer surface proteins in the functionality of different Lactobacillus strains

Autori
Raspović, Ivana

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Prehrambeno-biotehnološki fakultet

Mjesto
Zagreb

Datum
01.07

Godina
2013

Stranica
30

Mentor
Beganović, Jasna

Ključne riječi
in vitro adhesion; extracellular matrix; Lactobacillus; S-layer protein

Sažetak
The biological role of surface (S-) layer proteins, present at the cell wall, in just a several species of the genus Lactobacillus, has not yet been fully elucidated. The aim of this work was to screen different Lactobacillus strains for the presence of the S-layer surface proteins and to characterize these S-layer-expressing strains for their ability to adhere to extracellular matrix (ECM) proteins. Among 200 autochthonous species of lactic acid bacteria (LAB), strains isolated from different traditional fermented products, identified, characterised as probiotic and/or starter cultures and deposited in the Collection of Lactic Acid Bacteria (ZBMK) of Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology, Faculty of Food Technology and Biotechnology, were screened for the presence of S-layer proteins. SDS-PAGE was used as fast and efficient method to detect the presence of the putative S-layer proteins in different Lactobacillus strains. According to the SDS-PAGE analyses four different Lactobacillus strains exhibited S-layer proteins. The binding capability of the Lactobacillus strains varied among the Lactobacillus isolates and was specific depending of ECM protein. Removal of the intact S-layer proteins from the bacterial cell surface, by extraction with 5M guanidine hydrochloride, reduced the adhesion of Lactobacillus strains to fibronectin, and in some strains to collagen whereas, the adhesiveness to laminin increased with some strains.Binding tests showed that mostly selected Lactobacillus strains adhered in vitro to ECM proteins, in a proteinase K-sensitive manner.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

Napomena
Ovaj rad na napravljen u okviru integigranog diplomskog studija Sveučilišta u Zagrebu i Sveučilišta u Orléansu iz područja bioindustrijske tehnike

POVEZANOST RADA