Naslov
Grapevine shoot formation in vitro

Autori
Karoglan Kontić, Jasminka ; Mirošević, Nikola ; Jelaska, Sibila

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Vitis Special Issue - Proceedings of the 5th international Symposium on Grape Breeding / St. Martin/Pfalz - Lahti : Bundesforschungsanstalt fur Rebenzuchtung geilweilerhof, 1990, 466-466

Skup
Proceedings of the 5th international Symposium on Grape Breeding

Mjesto i datum
Sankt Martin, Njemačka, 12.09.1989. - 16.09.1989

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
vitis vinifera L.; tissue culture; micropropagation

Sažetak
In studies on in vitro propagation of Vitis vinifera L. (cvs Chardonnay, Pinot white, Sultanina, plavac mali and Plavac mali sivi, a spontaneous variant), shoot explants, 1-2 mm long, were grown on media with different types and concentrations of growth regulators, macronutrients and carbohydrates. Excised buds from canes, collected in march and held in water and in a controlled laboratory environment, were the source of explants. Very successful surface sterilization of buds was achieved with 1, 5% Izosan (chlorine product, Pliva, Zagreb) and 0, 01 % Tween 20 for 15 min, and rinsed in sterile distilled water (3 x 5 min.) Two basal media with macronutrients according to MURASHIGE and SKOOG (MS), full and half strength, and LLOYD and McCOWN (WPM) supplemented with 2% sucrose, 0, 8% Difco bacto agar and (mgl -1): 1 thiamine: HC1, 0, 5 pyridoxine: HC1, 2 glycine, 100 myo-inositol were tested. Cytokinin BA and auxins IAA and NAA in different concentrations and combinations were studied. The full strength MS medium with 1 mgl-1 BA and 0, 3 mgl-1 IAA gave the best results in establishing multiple shoot cultures. Effects of cultivar and time length in culture on multiplication rate, leaf shape deviation and vitrification was analysed.

Izvorni jezik
Engleski

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